- Discovery notes
- Open Access
Conservation of major and minor jelly-roll capsid proteins in Polinton (Maverick) transposons suggests that they are bona fide viruses
© Krupovic et al.; licensee BioMed Central Ltd. 2014
- Received: 10 March 2014
- Accepted: 24 April 2014
- Published: 29 April 2014
This article was reviewed by Lakshminarayan M. Iyer and I. King Jordan. For complete reviews, see the Reviewers’ Reports section.
Polintons (also known as Mavericks) and Tlr elements of Tetrahymena thermophila represent two families of large DNA transposons widespread in eukaryotes. Here, we show that both Polintons and Tlr elements encode two key virion proteins, the major capsid protein with the double jelly-roll fold and the minor capsid protein, known as the penton, with the single jelly-roll topology. This observation along with the previously noted conservation of the genes for viral genome packaging ATPase and adenovirus-like protease strongly suggests that Polintons and Tlr elements combine features of bona fide viruses and transposons. We propose the name ‘Polintoviruses’ to denote these putative viruses that could have played a central role in the evolution of several groups of DNA viruses of eukaryotes.
- Transposable elements
- Double jelly-roll fold
- Capsid proteins
- Virus evolution
Recently, an evolutionary connection has been established between eukaryotic transposons of the Polinton/Maverick family (hereafter Polintons) and the virophages [1, 2], a group of satellite viruses that parasitize the giant viruses of the Mimiviridae family [3–5]. Polintons are large (15–20 kb) DNA transposons that encode a set of conserved proteins, including protein-primed type B DNA polymerase (pPolB), RVE family integrase, FtsK-like ATPase, cysteine protease, and several uncharacterized proteins [6, 7]. These transposons are widely distributed in diverse unicellular and multicellular eukaryotes, attesting to their evolutionary success and/or ancient origin. We have previously pointed out that Polintons might encode a protein with the double jelly-roll fold found in the capsid proteins of viruses infecting hosts from all three domains of life . Here we investigate the possible viral nature of polintons and its implications.
The conserved polinton protein PY is a predicted major capsid protein
We next assessed the conservation of the Polinton MCP-coding genes and found that 46 of the 72 Polintons in the analyzed representative set encoded full-length MCPs (Additional file 1: Table S1), whereas in 13 other polintons the MCP-coding genes were split. Thus, more than 80% of the Polintons contain recognizable intact or fragmented MCP genes. This high conservation of the MCP proteins mirrors the conservation of the FtsK-like ATPase and cysteine protease (PRO), the two other viral-like proteins encoded by Polintons. The Polinton ATPase belongs to the family of genome-packaging ATPases of viruses with DJR MCPs [15, 16], whereas PRO is homologous to the virion maturation protease encoded by adenoviruses, virophages and many Nulceocytoplasmic Large DNA Viruses (NCLDV) of eukaryotes . The ATPase and PRO genes were identifiable in 83% and 74% of Polintons, respectively (Additional file 1: Table S1). Nine of the elements that did not encode MCPs also lacked the genes for both PRO and ATPase (Additional file 1: Table S1), suggestive of a concerted loss of genes implicated in the putative capsid morphogenesis. It therefore appears that among Polintons there is a continuity between viral and transposon states reminiscent of the well-studied Mu-like bacteriophages as well as metaviruses (also known as Ty3-gypsy retrotransposons) and pseudoviruses (also known as Ty1-copia retrotransposons) of eukaryotes that also combine the properties of both types of selfish elements [17–19].
Conserved polinton protein PX is a homolog of viral penton proteins
Viruses with the DJR MCPs use a common blueprint for capsid construction: pseudohexagonal trimers of the MCP are arrayed as triangular plates to form the icosahedral facets, whereas the five-fold vertices of the capsid are occupied by a different type of capsomer [8, 20–22]. In all the cases when structural information is available, the latter capsomers, called pentons in adenoviruses, display a single jelly-roll fold [14, 20, 23–25] and are typically encoded in the proximity of the MCP. For example, in all virophages penton protein homologs (minor capsid proteins) are encoded immediately upstream of the MCP . To investigate whether a corresponding protein is also conserved in Polintons, the penton protein sequence of Organic Lake Virophage (OLV8; ) was used as a seed in the Blastp search against the database of Polinton proteins. As a result, a match to the conserved Polinton protein PX  from Xenopus tropicalis was obtained (24% identity over 119 aa region; E = 3e-02). We further assessed the relationship between the penton proteins of virophages and PX proteins of Polintons by constructing multiple sequence alignment and inspecting the correspondence between the secondary structure elements among the aligned sequences (Additional file 3: Figure S2). Despite the overall low sequence similarity, which appears to be a general trend even among more closely related viruses , the correspondence of the secondary structure between the virophage and Polinton proteins is obvious. Further evidence indicating that PX represents the penton protein of Polintons comes from the conserved genomic location of the PX-coding gene which in Polintons is typically found either immediately upstream (e.g., P-9_NVi) or more commonly one gene away from the MCP gene. Taken together, the conserved sequences and secondary structures as well as positional gene conservation lead us to conclude that Polinton protein PX is a genuine penton protein.
Tlr elements also encode both the major and minor capsid proteins
Tlr elements found in the germline genome of the ciliate Tetrahymena thermophila represent a distinct type of large eukaryotic DNA transposons . They share with Polintons the genes for RVE family integrase and FtsK-like ATPase but instead of pPolB, encode a superfamily 1 helicase, which displays phylogenetic affinity to the homologous proteins from certain Megavirales as well as transpovirons [2, 28]. The presence of several viral-like genes, most notably the putative genome packaging ATPase, in the Tlr prompted us to investigate this element for the presence of the potential capsid protein gene(s). Indeed, we found that a protein (4Fp’) encoded by an open reading frame starting 78 codons upstream of the originally predicted gene 4F and completely encompassing it in frame, encodes a putative MCP of Tlr. When the 4Fp’ sequence was used as a query for the HHpred search, PBCV-1 VP54 was retrieved as the only significant hit with a probability P = 98.1. Alignment of the Tlr 4Fp’ sequence with those of the Polinton and PBCV-1 MCPs showed that the proteins align throughout their length and the secondary structure elements critical for the DJR fold are conserved (Figure 1). As in the case of P_1-DR MCP, the structural model of the 4Fp’ protein (Figure 2) displayed good stereochemical quality (Additional file 2: Figure S1), confirming that it is the DJR MCP of Tlr.
Furthermore, a Tblastn search against the Tlr sequence seeded with the putative Polinton penton proteins detected moderately significant similarity (E = 3e-04) between the predicted P-9_NVi penton protein and the product of a previously unannotated ORF of 227 codons located between the Tlr genes 7R and 1F . Reciprocally, the putative penton protein of Tlr retrieved the corresponding protein of P-9_NVi as the best hit from the database of all Polinton proteins. Taken together, these results strongly suggest that, similar to the Polintons, the transposable element Tlr encodes not only the putative viral genome-packaging ATPase but also the MCP and penton proteins.
A homologue of the Polinton MCP is encoded by the Phaeocystis globosavirus PgV-16T-associated virophage
Recently, a virophage-like element, PgVV, associated with the virus PgV-16T infecting Phaeocystis globosa has been described . PgVV has been reported to lack a capsid protein gene and to replicate as a linear plasmid . Given that many MCPs are highly divergent, we analyzed the PgVV for the presence of putative capsid protein genes by performing blastp searches with the Polinton MCPs as seeds. The best hit was obtained between the MCP of Polinton 3 (P-3_NV) from Nematostella vectensis and the PgVV protein PgVV_00012 (YP_008059897, 25% identity over 194 aa region, E = 0.01). This relationship was further validated by the evaluation of the multiple sequence alignment of PgVV_00012 with the MCPs of P-3_NV and PBCV-1 and subsequent assessment of the correspondence between their secondary structure elements. Like in the case of MCPs of polintons, the predicted secondary structure of PgVV_00012 was found to be consistent with the DJR topology (Additional file 4: Figure S3).
To better understand the relationship between the MCPs of Polintons, the Tlr element and the PgVV virophage, a maximum likelihood tree was constructed and rooted with the PBCV-1 MCP (Figure 2). According to this analysis, the MCP of the Tlr element forms an outgroup to the Polinton MCPs, suggesting that although Tlr and Polintons probably share a common origin, their divergence is an ancient event, in contrast to the previous suggestion that Tlr is a non-autonomous derivative of the Polintons . The MCP of PgVV formed a clade with the Polintons from N. vectensis (Figure 2), in agreement with the previous analysis that presented several lines of evidence of virophage evolution from Polintons . This conclusion contrasts the original proposal that virophages were ancestors of polintons that in large part rested on the assumption that virophages unlike Polintons are bona fide viruses . The demonstration that most of the Polintons encompass genes for two capsid proteins invalidates this argument.
Capsid proteins are among the viral hallmark proteins , and their presence distinguishes viruses from other types of mobile genetic elements [19, 31, 32]. Here we show that Polintons and Tlr elements, currently classified as non-viral transposable elements, encode two key proteins required for virion formation, the DJR MCP and the penton protein, i.e. the major and minor capsid proteins. This finding combined with previous observations that these elements also encode a typical viral genome-packaging ATPase and adenovirus-like protease (absent in Tlr) make a strong case that Polintons and Tlr elements comprise a group of genuine viruses that we propose to denote ‘Polintoviruses’. Polintoviruses might have played key roles in the evolution of DNA viruses of eukaryotes, in particular adenoviruses, virophages, and possibly the NCLDV. Identification of actively reproducing Polintoviruses is an important experimental challenge.
The Polinton nucleotide sequences were acquired from the Repbase Update database , which provides the consensus sequences of closely related transposons, thereby precluding redundancy. The dataset was further complemented with previously reported Polinton sequences [7, 34]. Polinton-like elements shorter than 10 kb were considered incomplete and removed, resulting in the final dataset of 72 non-redundant Polinton sequences. Distant homology detection was performed using HHpred . Fasta-formatted sequences of the major and minor capsid proteins of polintoviruses discussed in this study can be found in Additional file 5. Structural modelling was performed using Modeller v9.7 , essentially as described previously . The model was then verified for stereochemical consistency using ProSA-web . Protein sequences were aligned using Promals3D . For phylogenetic analysis, gapped columns (more than 30% of gaps) and columns with low information content were removed from the alignment . Maximum likelihood analysis was carried out by using PhyML 3.1 , with the WAG model of amino acid substitution, including a gamma law with 4 substitution rate categories.
Reviewer 1: Lakshminarayan M. Iyer (National Center for Biotechnology Information, National Library of Medicine, national Institute of Health)
The detection of the jelly roll capsids in various transposons is an important discovery and it clarifies the origins of the FtsK/HerA family ATPase-containing transposons that were previously suspected to be derivatives of virophages. They are now shown to be bonafide viruses. Understanding the biology of these elements is an exciting prospect for experimental studies. I have reproduced the results independently and confirm the sequence relationships mentioned in the text. Below are a few comments that the authors might find useful.
1. In general, it would be helpful if genbank ids or accession numbers of proteins described in the text are provided either in brackets next to the protein name (as was done in one instance) or in the supplementary table. The same applies to the alignment and tree figures in the main text and supplement, where they could either expand the species names or provide gis/accession numbers in the figure.
Authors’ response: In many cases, the major and minor capsid proteins were not properly annotated. Therefore, gis/accession numbers cannot be added. In the revised version of the manuscript we have included a new Additional file 5 which contains fasta-formatted sequences of all major and minor capsid proteins of polintoviruses discussed in this study. Furthermore, as suggested by the reviewer, we have expanded the species names in Figure 2 . In the other figures, the species names are indicated in the figure legends.
2. On page 3, the authors write “To further address the possibility that the PY proteins adopt the DJR fold, we used structural modeling followed by the assessment of the resultant model.” Structural modeling as done in this study is not really a reliable assessment tool for homology. Unrelated sequences have been wrongly fitted on to structures in other studies. The evidence provided in the previous paragraph is sufficient to establish a sequence relationship.
Authors’ response: This is a debatable issue. Certainly, unrelated sequences have been claimed to fit a particular structure on multiple occasions (just as unrelated sequences have been aligned to falsely claim homology). Nevertheless, we tend to believe that the quality of the model matters. This brief article, in any case, is not the place to plunge into such a general methodological debate, so we changed the language in question to make the text more neutral, in particular “To further examine the relationship between the PY proteins and viral capsid protein known to adopt the DJR fold, we used structural modeling…”
3. The relationship of PgVV_00012 to the capsid jelly rolls is not written in a convincing way. The authors should provide appropriate statistics.
Authors’ response: In the revised manuscript we have provided the E value for the hit between PgVV_00012 and the PY protein of P-3_NV (which is 0.01). We also pointed out that the predicted secondary structure of PgVV_00012 is consistent with the DJR topology, as is evident from the alignment shown in Additional file 4 : Figure S3.
4. The sequence of the Tlr penton protein is not provided. The supplementary alignment (S2) only shows one Tlr protein (which one is it?). The sequence might either be shown in an alignment or separately in the supplement.
Authors’ response: All protein sequences shown in the former Figure S2 (currently Additional file 3 : Figure S2) correspond to the penton proteins. Fasta-formatted sequences of all polinton proteins (including the one from Tlr) shown in the figure are now provided in the Additional file 5 .
5. It would help a reader if the major clades were labeled in Figure 2. This can be done either by using brackets or colored branches.
Authors’ response: Although we appreciate the suggestion, we think that further modifications to the figure would make it excessively crowded, especially now that the species names were expanded.
Reviewer 2: I.King Jordan (Department of Biology, Georgia Institute of Technology)
In this Discovery Note, Krupovic et al. make the case that two families of repetitive sequence elements, the Polintrons (Mavericks) and Tlr elements, formerly thought to be DNA transposons are more likely to be bona fide DNA viruses. The evidence in support of this assertion consists of deep sequence conservation and structural comparisons revealing that these families of genomic elements encode both major and minor viral capsid proteins, with the major capsid protein adopting the canonical jelly-roll structural topology. Such capsid proteins have previously been designated as features that distinguish viruses from transposons. This work reported here appears to be technically sound and the reasoning behind the authors’ argument for considering these mobile genetic element families is compelling.
My only suggestion is that they consider adding a figure to the paper that is a scheme illustrating the relationships between what they are designating as ‘Polintoviruses’ here and other families of DNA viruses in eukaryotes. Such an illustration could help to clarify the argument they make based on the tree topology shown in Figure 2 and may also underscore the continuity between viral and transposon states that they allude to in the manuscript.
Authors’ response: We certainly appreciate the importance of the relationships between Polintoviruses and other viral families but have to note that this is a major subject that goes far beyond the scope of the present brief note. We are preparing a new manuscript that will be dedicated to the implications of these relationships for the evolution of viruses.
MK was partly supported by the European Molecular Biology Organization (ASTF 82–2014).
DHB is supported by the Academy of Finland (grants 271413 and 272853 as well as Academy Professor funding grants 255342 and 256518). DHB is also grateful to the University of Helsinki for the support to EU ESFRI Instruct Centre.
EVK is supported by intramural funds of the US Department of Health and Human Services (to the National Library of Medicine).
- Fischer MG, Suttle CA: A virophage at the origin of large DNA transposons. Science. 2011, 332: 231-234. 10.1126/science.1199412.PubMedView ArticleGoogle Scholar
- Yutin N, Raoult D, Koonin EV: Virophages, polintons, and transpovirons: a complex evolutionary network of diverse selfish genetic elements with different reproduction strategies. Virol J. 2013, 10: 158-10.1186/1743-422X-10-158.PubMedPubMed CentralView ArticleGoogle Scholar
- Claverie JM, Abergel C: Mimivirus and its virophage. Annu Rev Genet. 2009, 43: 49-66. 10.1146/annurev-genet-102108-134255.PubMedView ArticleGoogle Scholar
- Desnues C, Boyer M, Raoult D: Sputnik, a virophage infecting the viral domain of life. Adv Virus Res. 2012, 82: 63-89.PubMedView ArticleGoogle Scholar
- Krupovic M, Cvirkaite-Krupovic V: Virophages or satellite viruses?. Nat Rev Microbiol. 2011, 9: 762-763. 10.1038/nrmicro2676.PubMedView ArticleGoogle Scholar
- Kapitonov VV, Jurka J: Self-synthesizing DNA transposons in eukaryotes. Proc Natl Acad Sci U S A. 2006, 103: 4540-4545. 10.1073/pnas.0600833103.PubMedPubMed CentralView ArticleGoogle Scholar
- Pritham EJ, Putliwala T, Feschotte C: Mavericks, a novel class of giant transposable elements widespread in eukaryotes and related to DNA viruses. Gene. 2007, 390: 3-17. 10.1016/j.gene.2006.08.008.PubMedView ArticleGoogle Scholar
- Krupovic M, Bamford DH: Virus evolution: how far does the double beta-barrel viral lineage extend?. Nat Rev Microbiol. 2008, 6: 941-948. 10.1038/nrmicro2033.PubMedView ArticleGoogle Scholar
- Söding J: Protein homology detection by HMM-HMM comparison. Bioinformatics. 2005, 21: 951-960. 10.1093/bioinformatics/bti125.PubMedView ArticleGoogle Scholar
- Nandhagopal N, Simpson AA, Gurnon JR, Yan X, Baker TS, Graves MV, Van Etten JL, Rossmann MG: The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus. Proc Natl Acad Sci U S A. 2002, 99: 14758-14763. 10.1073/pnas.232580699.PubMedPubMed CentralView ArticleGoogle Scholar
- Iyer LM, Balaji S, Koonin EV, Aravind L: Evolutionary genomics of nucleo-cytoplasmic large DNA viruses. Virus Res. 2006, 117: 156-184. 10.1016/j.virusres.2006.01.009.PubMedView ArticleGoogle Scholar
- Yutin N, Wolf YI, Raoult D, Koonin EV: Eukaryotic large nucleo-cytoplasmic DNA viruses: clusters of orthologous genes and reconstruction of viral genome evolution. Virol J. 2009, 6: 223-10.1186/1743-422X-6-223.PubMedPubMed CentralView ArticleGoogle Scholar
- Krupovic M, Bamford DH: Double-stranded DNA viruses: 20 families and only five different architectural principles for virion assembly. Curr Opin Virol. 2011, 1: 118-124. 10.1016/j.coviro.2011.06.001.PubMedView ArticleGoogle Scholar
- Zhang X, Sun S, Xiang Y, Wong J, Klose T, Raoult D, Rossmann MG: Structure of Sputnik, a virophage, at 3.5-A resolution. Proc Natl Acad Sci U S A. 2012, 109: 18431-18436. 10.1073/pnas.1211702109.PubMedPubMed CentralView ArticleGoogle Scholar
- Iyer LM, Makarova KS, Koonin EV, Aravind L: Comparative genomics of the FtsK-HerA superfamily of pumping ATPases: implications for the origins of chromosome segregation, cell division and viral capsid packaging. Nucleic Acids Res. 2004, 32: 5260-5279. 10.1093/nar/gkh828.PubMedPubMed CentralView ArticleGoogle Scholar
- Strömsten NJ, Bamford DH, Bamford JK: In vitro DNA packaging of PRD1: a common mechanism for internal-membrane viruses. J Mol Biol. 2005, 348: 617-629. 10.1016/j.jmb.2005.03.002.PubMedView ArticleGoogle Scholar
- Krupovic M, Prangishvili D, Hendrix RW, Bamford DH: Genomics of bacterial and archaeal viruses: dynamics within the prokaryotic virosphere. Microbiol Mol Biol Rev. 2011, 75: 610-635. 10.1128/MMBR.00011-11.PubMedPubMed CentralView ArticleGoogle Scholar
- Mizuuchi K, Craigie R: Mechanism of bacteriophage mu transposition. Annu Rev Genet. 1986, 20: 385-429. 10.1146/annurev.ge.20.120186.002125.PubMedView ArticleGoogle Scholar
- Koonin EV, Dolja VV: Virus world as an evolutionary network of viruses and capsid-less selfish elements. Microbiol Mol Biol Rev. 2014, In pressGoogle Scholar
- Abrescia NG, Grimes JM, Kivelä HM, Assenberg R, Sutton GC, Butcher SJ, Bamford JK, Bamford DH, Stuart DI: Insights into virus evolution and membrane biogenesis from the structure of the marine lipid-containing bacteriophage PM2. Mol Cell. 2008, 31: 749-761. 10.1016/j.molcel.2008.06.026.PubMedView ArticleGoogle Scholar
- Benson SD, Bamford JK, Bamford DH, Burnett RM: Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat protein structures. Cell. 1999, 98: 825-833. 10.1016/S0092-8674(00)81516-0.PubMedView ArticleGoogle Scholar
- Stewart PL, Burnett RM, Cyrklaff M, Fuller SD: Image reconstruction reveals the complex molecular organization of adenovirus. Cell. 1991, 67: 145-154. 10.1016/0092-8674(91)90578-M.PubMedView ArticleGoogle Scholar
- Abrescia NG, Cockburn JJ, Grimes JM, Sutton GC, Diprose JM, Butcher SJ, Fuller SD, San Martin C, Burnett RM, Stuart DI, Bamford DH, Bamford JK: Insights into assembly from structural analysis of bacteriophage PRD1. Nature. 2004, 432: 68-74. 10.1038/nature03056.PubMedView ArticleGoogle Scholar
- Veesler D, Ng TS, Sendamarai AK, Eilers BJ, Lawrence CM, Lok SM, Young MJ, Johnson JE, Fu CY: Atomic structure of the 75 MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography. Proc Natl Acad Sci U S A. 2013, 110: 5504-5509. 10.1073/pnas.1300601110.PubMedPubMed CentralView ArticleGoogle Scholar
- Zubieta C, Schoehn G, Chroboczek J, Cusack S: The structure of the human adenovirus 2 penton. Mol Cell. 2005, 17: 121-135. 10.1016/j.molcel.2004.11.041.PubMedView ArticleGoogle Scholar
- Yau S, Lauro FM, DeMaere MZ, Brown MV, Thomas T, Raftery MJ, Andrews-Pfannkoch C, Lewis M, Hoffman JM, Gibson JA, Cavicchioli R: Virophage control of antarctic algal host-virus dynamics. Proc Natl Acad Sci U S A. 2011, 108: 6163-6168. 10.1073/pnas.1018221108.PubMedPubMed CentralView ArticleGoogle Scholar
- Wuitschick JD, Gershan JA, Lochowicz AJ, Li S, Karrer KM: A novel family of mobile genetic elements is limited to the germline genome in Tetrahymena thermophila. Nucleic Acids Res. 2002, 30: 2524-2537. 10.1093/nar/30.11.2524.PubMedPubMed CentralView ArticleGoogle Scholar
- Desnues C, La Scola B, Yutin N, Fournous G, Robert C, Azza S, Jardot P, Monteil S, Campocasso A, Koonin EV, Raoult D: Provirophages and transpovirons as the diverse mobilome of giant viruses. Proc Natl Acad Sci U S A. 2012, 109: 18078-18083. 10.1073/pnas.1208835109.PubMedPubMed CentralView ArticleGoogle Scholar
- Santini S, Jeudy S, Bartoli J, Poirot O, Lescot M, Abergel C, Barbe V, Wommack KE, Noordeloos AA, Brussaard CP, Claverie JM: Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes. Proc Natl Acad Sci U S A. 2013, 110: 10800-10805. 10.1073/pnas.1303251110.PubMedPubMed CentralView ArticleGoogle Scholar
- Koonin EV, Senkevich TG, Dolja VV: The ancient Virus World and evolution of cells. Biol Direct. 2006, 1: 29-10.1186/1745-6150-1-29.PubMedPubMed CentralView ArticleGoogle Scholar
- Krupovic M, Bamford DH: Order to the viral universe. J Virol. 2010, 84: 12476-12479. 10.1128/JVI.01489-10.PubMedPubMed CentralView ArticleGoogle Scholar
- Raoult D, Forterre P: Redefining viruses: lessons from Mimivirus. Nat Rev Microbiol. 2008, 6: 315-319. 10.1038/nrmicro1858.PubMedView ArticleGoogle Scholar
- Jurka J, Kapitonov VV, Pavlicek A, Klonowski P, Kohany O, Walichiewicz J: Repbase Update, a database of eukaryotic repetitive elements. Cytogenet Genome Res. 2005, 110: 462-467. 10.1159/000084979.PubMedView ArticleGoogle Scholar
- Dupuy C, Periquet G, Serbielle C, Bezier A, Louis F, Drezen JM: Transfer of a chromosomal Maverick to endogenous bracovirus in a parasitoid wasp. Genetica. 2011, 139: 489-496. 10.1007/s10709-011-9569-x.PubMedView ArticleGoogle Scholar
- Marti-Renom MA, Stuart AC, Fiser A, Sanchez R, Melo F, Sali A: Comparative protein structure modeling of genes and genomes. Annu Rev Biophys Biomol Struct. 2000, 29: 291-325. 10.1146/annurev.biophys.29.1.291.PubMedView ArticleGoogle Scholar
- Roux S, Enault F, Bronner G, Vaulot D, Forterre P, Krupovic M: Chimeric viruses blur the borders between the major groups of eukaryotic single-stranded DNA viruses. Nat Commun. 2013, 4: 2700-PubMedView ArticleGoogle Scholar
- Wiederstein M, Sippl MJ: ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Res. 2007, 35: W407-W410. 10.1093/nar/gkm290.PubMedPubMed CentralView ArticleGoogle Scholar
- Pei J, Kim BH, Grishin NV: PROMALS3D: a tool for multiple protein sequence and structure alignments. Nucleic Acids Res. 2008, 36: 2295-2300. 10.1093/nar/gkn072.PubMedPubMed CentralView ArticleGoogle Scholar
- Yutin N, Makarova KS, Mekhedov SL, Wolf YI, Koonin EV: The deep archaeal roots of eukaryotes. Mol Biol Evol. 2008, 25: 1619-1630. 10.1093/molbev/msn108.PubMedPubMed CentralView ArticleGoogle Scholar
- Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O: New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010, 59: 307-321. 10.1093/sysbio/syq010.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.