Figure 2From: Proteomic and bioinformatic analysis of epithelial tight junction reveals an unexpected cluster of synaptic moleculesImmunofluorescence staining of tight junction proteins. (A) Protocol for visualization of tight junction proteins in monolayer of cells grown on filter support (see Methods for details). (B) Confocal images of T84 cells scanned at 1 μm intervals. Occludin (green) and PKC zeta (red) are localized to the apical tight junction domain. Merged images show both proteins appearing and disappearing together in the same confocal planes.Back to article page