Fig. 5
From: OTUD5 promotes the growth of hepatocellular carcinoma by deubiquitinating and stabilizing SLC38A1
OTUD5 binds to SLC38A1. (A) Label-free mass spectrometry was performed on HCCLM3 cells with or without OTUD5 knockdown to identify differentially expressed proteins. (B) The binding between endogenous OTUD5 and SLC38A1 in HCCLM3 cells was demonstrated by co-IP. (C) The flag-tagged OTUD5 plasmid was transduced into HEK293T cells. Proteins were precipitated with a FLAG-tagged antibody, and SLC38A1 was detected by Western blotting. (D) HCCLM3 and Huh-7 cells that were transduced with OTUD5 shRNAs or NT shRNA were subjected to Western blotting for SLC38A1 and OTUD5 expression. (E) HepG2 and Hep3B cells that were transduced with an empty vector (EV) or an OTUD5 expression vector were subjected to Western blotting for SLC38A1 and OTUD5 expression. *P < 0.05