Fig. 3

Enforced expression of ZNF692 promotes cell proliferation, migration, and invasion. (A-C) Ectopic expression of ZNF692 was determined by qRT-PCR and western blot assays. (D-E) CCK-8 assay was performed to compare cell proliferation ability in cells stably overexpressing ZNF692 and control cells. (F-G) Representative images and quantitative analysis of colony formation in cells stably overexpressing ZNF692 and control cells. (H) The proportion of Edu positive cell was significantly increased in osteosarcoma cells stably overexpressing ZNF692. Scale bar, 200 μm. (I-K) Representative images and quantitative analysis of cell migration based on wound healing assay. Scale bar, 200 μm. (L-M) Enforced expression of ZNF692 increased cell invasion ability based on transwell assay. Student’s t-test and one-way ANOVA were performed to analyze differences between groups. All data are presented as means ± standard deviations (SD). *P < 0.05, **P < 0.01, ***P < 0.001