Fig. 1
From: Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging
Expression and localization of AMPK in mouse oocytes. (A) The AMPK protein expression in metaphase I and metaphase II of mouse oocytes by western blotting. 200 oocytes were used for each sample. (B) The localization of AMPK during mouse oocyte maturation. AMPK localized in the cytoplasm of mouse oocytes from GV and MI stage, while there was no AMPK signal in the negative control groups. Green, AMPK; blue, DNA. Bar = 20 μm. (C) The AMPK protein expression in metaphase II of mouse oocytes during aging. 150 oocytes were used for each sample. **P < 0.05. (D) The localization of AMPK during mouse oocyte aging. In the aged oocytes, there was no difference for the AMPK localization, however, the fluorescence signals were much weaker than the fresh oocytes. Green, AMPK; blue, DNA. Bar = 20 μm. (E) The rate of relative intensity of AMPK. **P < 0.05