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Fig. 3 | Biology Direct

Fig. 3

From: LINC00909 up-regulates pluripotency factors and promotes cancer stemness and metastasis in pancreatic ductal adenocarcinoma by targeting SMAD4

Fig. 3

LINC00909 is associated with the MAPK/JNK pathway and pluripotency factors in pancreatic ductal adenocarcinoma (PDAC). (A) Gene Ontology (GO) enrichment analysis of LINC00909 based on the NGS database. (B) Top 15-enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of LINC00909 based on the NGS database. (C) Gene set enrichment analysis (GSEA) of the NGS database. (D) The expression of factors associated with stemness and metastasis was analyzed by NGS using PANC-1/Vector and PANC-1/LINC00909-OE cells. These factors are shown in the heatmap. (E–G) Western blotting analysis was conducted to detect the protein expression of JNK phospho-JNK, c-JUN, phospho-JUN, TWIST, and SNAIL in LINC00909-overexpressing cells (E) and LINC00909-knockdown cells (F, G). All *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. P-values were assessed using ANOVA and two-tailed t-tests, followed by Dunnett’s tests for multiple comparisons in (D)

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Biology Direct

ISSN: 1745-6150

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