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Fig. 2 | Biology Direct

Fig. 2

From: LINC00909 up-regulates pluripotency factors and promotes cancer stemness and metastasis in pancreatic ductal adenocarcinoma by targeting SMAD4

Fig. 2

LINC00909 promotes the proliferation and migration of pancreatic ductal adenocarcinoma (PDAC) cells. (A) RT-qPCR showed that LINC00909 was highly expressed in PANC-1 and AsPC-1 cells among PDAC cell lines. (B) Identification of the cytoplasmic location of LINC00909 in AsPC-1, PANC-1, and CAPAN-2 cells using FISH. (C) The efficiency of LINC00909 overexpression in PANC-1 cells was assessed using RT-qPCR. (D) The effects of LINC00909 overexpression on cell proliferation and viability were verified using CCK-8 assays. (E, F) The efficiency of LINC00909 knockdown in PANC-1 and AsPC-1 cells was detected by RT-qPCR. (G, H) The effects of LINC00909 knockdown on cell proliferation and viability were verified using CCK-8 assays. (I–K) The effect of LINC00909 overexpression and knockdown on colony formation was investigated in PDAC cell lines. (L–N) Representative images of Transwell assays after LINC00909 overexpression and knockdown in PDAC cell lines. All *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. P-values were assessed using ANOVA and two-tailed t-tests, followed by Dunnett’s tests for multiple comparisons in (A) and (C–N). All data represent the means ± SD from 3 independent assays

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Biology Direct

ISSN: 1745-6150

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