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Fig. 4 | Biology Direct

Fig. 4

From: Unlocking hepatocellular carcinoma aggression: STAMBPL1-mediated TRAF2 deubiquitination activates WNT/PI3K/NF-kb signaling pathway

Fig. 4

A qRT-PCR validation of the overexpression efficiency of STAMBPL1 in HCCLM3 cells. B qRT-PCR validation of the overexpression efficiency of STAMBPL1 in Hep3B cells. C, D Western blot validation of the overexpression efficiency of STAMBPL1 in HCCLM3 cell and Hep3B cell. E The CCK-8 experiment to assess the proliferation efficiency between the vector group and the STAMBPL1 over-expressed group in HCCLM3 and Hep3B cell. F The colony formation assay to examine the proliferation efficiency of the vector group and the STAMBPL1 over-expressed group in HCCLM3 and Hep3B cell. G The EdU assay to assess the proliferation efficiency of the vector group and the STAMBPL1 over-expressed group in HCCLM3 and Hep3B cell. H Western blot analysis to detect the protein expression levels of Cyclin D1, P21, PCNA, and Ki-67 in the vector group and the STAMBPL1 over-expressed group of HCCLM3 and Hep3B cells. I, J The transwell assay to assess the migration and invasion efficiency between the vector group and the STAMBPL1 over-expressed group of HCCLM3 and Hep3B cells. K, L Wound healing assay to assess the migration efficiency of the vector group and the STAMBPL1 over-expressed group of HCCLM3 and Hep3B cells. M Western blot analysis to detect the protein expression levels of E-cadherin, N-cadherin, MMP3, Vimentin and Snail in the vector group and the STAMBPL1 over-expressed group of HCCLM3 and Hep3B cells

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