Fig. 6

GPR137 loss declines AGS cell malignancy but potentiates Hippo activity. A Protein expression examination of GPR137 in GPR137-knockout (GPR137-KO) AGS cells or control AGS cells. One representative of 3 independent blots is shown. B CCK-8 assays of GPR137-knockout AGS cells or control cells at different periods. **p < 0.01; error bar, SD. N = 3. C Wound healing assays of GPR137-knockout (GPR137-KO) AGS cells or control cells at 24 h. Bar = 100 μm. N = 3. D Statistical analysis of unoccupied area in (C). **p < 0.01; error bar, SD. E Matrigel invasion assays of GPR137-knockout (GPR137-KO) AGS cells or control cells at 24 h. Bar = 100 μm. N = 3. F Quantitative analysis of (E). **p < 0.01; error bar, SD. G Colony formation assays of GPR137-knockout (GPR137-KO) AGS cells or control cells. N = 3. H Quantitative analysis of (G). **p < 0.01; error bar, SD. I Protein levels of pMST1 and MST1 in GPR137-knockout (GPR137-KO) AGS cells or control cells. One representative of 3 independent blots is shown. J Protein levels of pTAZ, TAZ, pYAP and YAP in GPR137-knockout (GPR137-KO) AGS cells or control cells. One representative of 3 independent blots is shown. K Nucleo-cytoplasmic separation assays in GPR137-knockout AGS (GPR137-KO) cells or control cells, and protein levels of YAP and TAZ were detected. Proteins in nucleus (upper) and in cytoplasm (lower). One representative of 3 independent blots is shown. L, M qRT-PCR assays for mRNA levels of CTGF (L) and CYR61 (M) in GPR137-knockout (GPR137-KO) AGS cells or control cells. **p < 0.01; error bar, SD. N = 3. N Immunofluorescent staining for HA-derived signal in GPR137-knockout (GPR137-KO) AGS cells or control cells transfected with HA-TAZ-S89A (upper) or HA-YAP-S127A (lower). Nuclei were stained with DAPI. Bar = 5 μm. N = 3