Fig. 5
From: GPR137 inactivates Hippo signaling to promote gastric cancer cell malignancy
GPR137 suppresses Hippo signaling by MST. A Protein levels of pMST1, MST1, pLATS1, LATS1, pLATS2 and LATS2 in AGS cells transfected with GPR137 (GPR137, +) or an empty vector (GPR137, -) for 24 h. One representative of 3 independent blots is shown. B Protein levels of pTAZ, TAZ, pYAP, YAP, CTGF and CYR61 in AGS cells transfected with GPR137 (GPR137, +) or an empty vector (GPR137, -) for 24 h. One representative of 3 independent blots is shown. C Co-immunoprecipitation of HA-tagged GPR137 and Flag-tagged MST1 in HEK293T cells. IP: Flag; WB: HA. N = 3. D Co-immunoprecipitation of endogenous GPR137 and MST1 in AGS cells. IP: MST1; WB: GPR137. IgG was used as a negative control. One representative of 3 independent blots is shown. E Co-immunoprecipitation of endogenous MST1 and LATS1 in the presence of GPR137 in AGS cells. IP: MST1; WB: LATS1. IgG was used as a negative control. One representative of 3 independent blots is shown. F Co-immunoprecipitation of endogenous MST1 and LATS1 in the presence of XMU-MP-1 in AGS cells. IP: MST1; WB: LATS1. IgG was used as a negative control. One representative of 3 independent blots is shown. G Protein expression examination of MST1 and MST2 in MST1/2-knockout (MST1/2-KO) AGS cells or control AGS cells. One representative of 3 independent blots is shown. H Nucleo-cytoplasmic separation assays in MST1/2-knockout AGS (MST1/2-KO) cells or control cells, and protein levels of YAP and TAZ were detected. Proteins in nucleus (left) and in cytoplasm (right). One representative of 3 independent blots is shown. I Nucleo-cytoplasmic separation assays in GPR137-transfected AGS (GPR137, +) cells or control cells (GPR137, -), and protein levels of YAP and TAZ were detected. Proteins in nucleus (upper) and in cytoplasm (lower). One representative of 3 independent blots is shown. J TEAD-luciferase assays in MST1/2-knockout (MST1/2-KO) AGS cells or control AGS cells transfected with GPR137 or an empty vector (control). **p < 0.01; error bar, SD. N = 3. K TEAD-luciferase assays in MST1/2-knockout (MST1/2-KO) AGS cells or control AGS cells transfected with GPR137 shRNA or control scrambled shRNA. **p < 0.01; error bar, SD. N = 3