Fig. 4

GPR137 inhibits Hippo signaling activity. A TEAD-luciferase assays in AGS cells transfected with GPR137 or an empty vector (control) for 24 h. **p < 0.01; error bar, SD. N = 3. B Protein levels of GPR137 in AGS cells transfected with GPR137 shRNA (shGPR137, +) or control scrambled shRNA (shGPR137, -) for 72 h. One representative of 3 independent blots is shown. C TEAD-luciferase assays in AGS cells transfected with GPR137 shRNA or control scrambled shRNA and culture for 72 h. **p < 0.01; error bar, SD. N = 3. D, E qRT-PCR assays for mRNA levels of CTGF (D) and CYR61 (E) in AGS cells transfected with GPR137 or an empty vector (control) for 24 h. **p < 0.01; error bar, SD. N = 3. F Protein levels of GPR137 in SGC-7901 cells transfected with GPR137 (GPR137, +) or an empty vector (GPR137, -) for 24 h. One representative of 3 independent blots is shown. G, H) qRT-PCR assays for mRNA levels of CTGF G and CYR61 (H) in SGC-7901 cells transfected with GPR137 or an empty vector (control) for 24 h. **p < 0.01; error bar, SD. N = 3. I, J qRT-PCR assays for mRNA levels of YAP (I) and TAZ (J) in AGS cells transfected with GPR137 or an empty vector (control) for 24 h. Error bar, SD. N = 3. K, L GEPIA database displayed the correlation between GPR137 and CTGF (K) as well as GPR137 and CYR61 (L) in STAD