Fig. 2

GPR137 promotes AGS cell malignancy. A CCK-8 assays of AGS cells transfected with GPR137 or an empty vector (control) and cultured for different periods. **p < 0.01; error bar, SD. N = 3. B Wound healing assays of AGS cells transfected with GPR137 or an empty vector (control) at 24 h. Bar = 100 μm. N = 3. C Statistical analysis of unoccupied area in (B). **p < 0.01; error bar, SD. D Matrigel invasion assays of AGS cells transfected with GPR137 or an empty vector (control) for 24 h. Bar = 100 μm. N = 3. E Quantitative analysis of (D). **p < 0.01; error bar, SD. F Colony formation assays of AGS cells transfected with GPR137 or an empty vector (control). N = 3. G Quantitative analysis of (F). **p < 0.01; error bar, SD. H Nude mice with xenografts derived from AGS cells infected with GPR137-expressing lentiviruses (GPR137) or control viruses (Control). I, J Tumor weight (I) and tumor volume (J) in (H). N = 6; **p < 0.01; error bar, SD