Fig. 9

MCM4 expression is upregulated by PGK1. a The ranking of differential gene expression was performed using RNA-seq data. b The detection of mRNA expression for specific genes was accomplished through qRT-PCR. c The examination of the correlation between PGK1 and chosen genes was conducted by utilizing the TCGA database. d-f The assessment of MCM4 mRNA expression in NSCLC tissues was carried out via the utilization of TCGA, GENT2, and GEO databases. g The Western blotting technique was employed to validate the protein expression of MCM4. h Analysis of MCM4 expression by qRT-PCR and Western blotting. i-k The CCK-8, colony formation, and Transwell assays were utilized to independently measure cell growth, migration, and invasion across different groups. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001