Fig. 5
From: The oncogenic role and regulatory mechanism of PGK1 in human non-small cell lung cancer
PGK1 interacts with and is O-GlcNAcylated by OGT. a The evaluation of OGT mRNA levels in both tumor and normal tissues of NSCLC patients from the TCGA, GENT2, and GEO databases. b The detection of OGT mRNA expression was performed using qRT-PCR. c The analysis of OGT protein expression was conducted through Western blotting. d Co-IP was employed to investigate the interaction between PGK1 and OGT. e The assessment of cell proliferation was accomplished by means of the CCK-8 assay. f The monitoring of cell colony-forming ability was executed using the colony formation assay. g The measurement of cell migration and invasion was carried out utilizing the Transwell assay. *P < 0.05, **P < 0.01, and ***P < 0.001