Fig. 3
From: The oncogenic role and regulatory mechanism of PGK1 in human non-small cell lung cancer
Knockdown of PGK1 suppresses NSCLC growth and metastasis. a The expression of PGK1 mRNA was detected using qRT-PCR. b The expression of PGK1 protein was analyzed through Western blotting. c Cell proliferation was assessed via the CCK-8 assay. d The Colony formation assay was utilized to evaluate the cell colony-forming ability. e The Transwell assay was employed to measure cell migration and invasion. f Flow cytometry was conducted to examine cell cycle distribution. g Flow cytometry was performed to monitor cell apoptosis. h Representative images of xenograft tumors in nude mice. i Assessment of the size and weight of xenograft tumors in nude mice. j Immunohistochemical examination of PGK1 and Ki-67 expression in tumors dissected from nude mice. *P < 0.05, **P < 0.01, and ***P < 0.001