Fig. 6

circLRIG1 regulates LRIG1 expression by sponging miR-214-3p. A Venn diagram showing the overlap of miRNAs targeted by circLRIG1 and LRIG1 in bladder carcinoma cells. B RNA pull-down assay using circLRIG1 and Oligo probes was performed in UMUC3 cells. C Potential targets of wild-type and mutant circLRIG1 or LRIG1 on miR-214-3p. D Relationship between circLRIG1 and miR-214-3p was determined using the luciferase reporter assay. E Relationship between miR-214-3p and LRIG1 was determined using the luciferase reporter assay. F Relationship between circLRIG1 and miR-214-3p or miR-214-3p and LRIG1 was determined using the RNA pull-down assay. G Relationship between circLRIG1 and miR-214-3p or miR-214-3p and LRIG1 was determined using the RNA immunoprecipitation (RIP) assay. H Protein level of LRIG1 in bladder carcinoma cell lines (UMUC3 and T24) was determined by Western blot assay after transfection with indicated constructs (miR-214-3p mimics, mimics control). I Protein level of LRIG1 in bladder carcinoma cell lines (UMUC3 and T24) was determined by Western blot assay after transfection with indicated constructs (OE-vector, OE-circLRIG1, OE-circLRIG1 + miR-214-3p mimics). J QRT-PCR analysis of miR-214-3p mRNA levels in 90 pairs of bladder carcinoma tissues and adjacent noncancerous specimens. K The correlation between the expression of miR-214-3p and circLRIG1 as well as LRIG1 in bladder carcinoma tissues was analyzed using Pearson’s correlation coefficient. The experiment was performed in triplicate. “**” represents P < 0.01