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Fig. 4 | Biology Direct

Fig. 4

From: Circ_MAPK9 promotes STAT3 and LDHA expression by silencing miR-642b-3p and affects the progression of hepatocellular carcinoma

Fig. 4

Circ_MAPK9 mediates HCC cell proliferation by regulating miR-642b-3p. A and B, RNA-FISH and qRT-PCR analysis of the localization of circ_MAPK9 in

SMMC-7721 cells. C and D, The expression levels of MAPK9 mRNA and protein after down-regulated circ_MAPK9 expression, detected by qRT-PCR and Western blot. E, Schematic representation of the binding sites between miR-642b-3p and WT circ_MAPK9 3’UTR. Comparison of the luciferase activity of circ_MAPK9 3’UTR after treatment with miR-642b-3p mimics in HEK293T cells. F, The effect of circ_MAPK9 knockdown on miR-642b-3p expression in SMMC-7721 and SK-Hep1 cells analyzed by qRT-PCR. G, Cell proliferation after transfection with circ_MAPK9 siRNA and/or miR-642b-3p inhibitor in SMMC-7721 and SK-Hep1 cells, analyzed by EdU and colony formation assay. *P < 0.05

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Biology Direct

ISSN: 1745-6150

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