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Fig. 2 | Biology Direct

Fig. 2

From: Circ_MAPK9 promotes STAT3 and LDHA expression by silencing miR-642b-3p and affects the progression of hepatocellular carcinoma

Fig. 2

Knockdown of circ_MAPK9 inhibits the proliferation, migration and invasion of HCC cells, but induces cellular apoptosis. A, The knockdown efficiency of circ_MAPK9 in SMMC-7721 and SK-Hep1 cells, determined by qRT-PCR. B–C, Circ_MAPK9 knockdown significantly reduced the proliferative capacity of SMMC-7721 and SK-Hep1 cells, determined by CCK8 and colony formation experiments. D-E, Circ_MAPK9 knockdown suppressed HCC cells migration and invasion, evaluated by the transwell and Boyden assay (magnification: ×200). F, Cells with silenced circ_MAPK9 expression were stained with a combination of Annexin V and PI and analyzed by Flow cytometric assay (FACS). Cells positive for Annexin V staining were counted as apoptotic cells, and the percentage of apoptotic cells is shown. G, The expression of apoptosis related proteins, measured by Western blotting assay. GAPDH was used as a loading control (*P < 0.05)

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