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Fig. 1 | Biology Direct

Fig. 1

From: Circ_MAPK9 promotes STAT3 and LDHA expression by silencing miR-642b-3p and affects the progression of hepatocellular carcinoma

Fig. 1

Identification of the circular structure of circ_MAPK9 and its expression in HCC cells and liver cancer tissues. A, The expression level of circ_MAPK9 in HCC patient data from GSE97332 dataset. B, The existence of circ_MAPK9 was validated in SMMC-7721 cell line by RT-PCR. Convergent primer amplified circ_MAPK9 fragments from cDNA and gDNA. Divergent primers amplified circ_MAPK9 fragment from cDNA, but not from gDNA. GAPDH was used as a negative control. The red arrow represented the “head-to-tail” splicing site of circ_MAPK9 according to Sanger sequencing. C, The expression level of circ_MAPK9 and linear MAPK9 mRNA in SMMC-7721 and SK-Hep1 cells treated with RNase R, which were normalized to mock treatment, determined by qRT-PCR. D, The expression levels of circ_MAPK9 in normal hepatocytes WRL68 and HCC cell lines. E, Circ_MAPK9 expression in 54 paired clinical HCC tissues, detected by qRT-PCR. **P < 0.01

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Biology Direct

ISSN: 1745-6150

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