Fig. 5

ESCC cell progression promoted by PHF5A was dependent on VEGFA. (A) Relative mRNA and (B) protein levels of PHF5A and VEGFA in Eca-109 cells overexpressed PHF5A, VEGFA knockdown, or simultaneous PHF5A overexpression and VEGFA knockdown were detected by qPCR and WB assays respectively. GAPDH was used as the internal control. (C) Celigo cell count assay and (D) colony formation assays were used to evaluate growth of Eca-109 cells. (E) The roles of PHF5A/VEGFA on Eca-109 cell apoptosis were assessed by flow cytometry. (F) Transwell and (G) wound-healing assays of PHF5A overexpressing/VEGFA silencing were performed to reveal their regulatory effects on Eca-109 cell migration. The experiments were repeated three times independently. *p < 0.05, **p < 0.01, ***p < 0.001