Fig. 2
From: PHF5A promotes esophageal squamous cell carcinoma progression via stabilizing VEGFA
PHF5A depletion inhibited cell growth and migration with apoptotic induction and cell cycle arrest. (A) PHF5A knockdown efficiency was evaluated at mRNA and protein levels by qPCR and (B) western blotting, respectively. GAPDH was used as the internal reference. (C) Celigo cell count assay was used for detecting the cell proliferation within five successive days. (D) Esophageal cancer cell apoptosis and (E) cell cycle distribution was analyzed by flow cytometer after PHF5A silence. (F) Wound-healing assay and (G) transwell assay were performed to determine cell migration upon PHF5A knockdown. The representative images were randomly selected from at least 3 independent experiments. **p < 0.01, ***p < 0.001