Fig. 7

NRF1 promotes HCC growth in vivo. Lentivirus was used to establish stable NRF1 knockdown Huh7 cell lines and tumor growth ability was assessed with subcutaneous xenograft model. A The image of subcutaneous xenograft tumors were shown. B The tumor volume was measured every three days. C The mass of the subcutaneous xenograft tumors was weighed after excision. D Representative images of IHC staining and the IHC scores of NRF1, LPCAT1, p-ERK1/2, p-CREB, Ki67 and Vimentin of the tumors. Scale bar: 50 μm. E NRF1, LPCAT1, p-ERK1/2, ERK1/2, p-CREB, CREB, PCNA and Vimentin were analyzed with Western blot. Bar graphs showed the quantitative results. *p < 0.05, **p < 0.01, ***p < 0.001