Fig. 6

LPCAT1 mediates the oncogenic potential of NRF1 by modulating the ERK1/2-CREB signaling. A-C The proliferation ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by CCK8 (A-B) and colony formation assay (C). (D-F) The migration and invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by transwell (D-E) and wound healing assay (F). (G) The cell cycle and EMT process related genes expression were determined by Western blot in Huh7 and MHCC97H after NRF1 knockdown and LPCAT1 overexpression. H GSEA using KEGG MAPK pathway gene set between high NRF1 group and low NRF1 group in TCGA-LIHC dataset. I The activation of ERK1/2-CREB pathway and NRF1, LPCAT1 protein levels were analysed by Western blot after NRF1 knockdown and LPCAT1 overexpression. J The proliferation ability of Huh7 cells after NRF1 knockdown and PD184352 treatment was determined by CCK8 assay. K The invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and PD184352 treatment was determined by transwell assay. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant