Fig. 5

LPCAT1 and NRF1 constitute a positive feedback loop through activating the ERK1/2-CREB pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B-C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot (B) and qRT-PCR (C), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E–F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment (E) or CREB knockdown (F) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. *p < 0.05, ** p  < 0.01, *** p  < 0.001