Fig. 6

The tumor-promoting function of KIAA1429 depends on ENO1. A qRT-PCR and Western blotting were performed to determine ENO1 expression in differently treated cells. n = 3 independent experiments. B, C Cell proliferation was determined by CCK-8 assay and colony formation assay. n = 3 independent experiments. D, E Cell invasion and migration were determined by wound-healing migration assay and Transwell invasion assay. n = 3 independent experiments. F Curves show the volume and growth of subcutaneous xenografts of OC cells in BALB/c nude mice after cell inoculation for 20 days. n = 5 independent experiments. G IHC and IF staining for Ki-67 and TUNEL assays in BALB/c nude mice after cell inoculation 20 days. n = 5 independent experiments. H The fluorescence intensity values and the number of intraperitoneal metastatic foci in intraperitoneal metastasis BALB/c nude mice after cell inoculation for 30 days are shown. n = 5 independent experiments. I The number of lung metastatic foci in lung metastasis BALB/c nude mice after cell inoculation 40 days are shown. n = 5 independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001