Skip to main content
Fig. 5 | Biology Direct

Fig. 5

From: N6-methyladenosine methyltransferase KIAA1429 promoted ovarian cancer aerobic glycolysis and progression through enhancing ENO1 expression

Fig. 5

KIAA1429-mediated stabilization of ENO1 mRNA relies on m6A modification. A qRT-PCR and Western blotting were performed to determine ENO1 expression in differently treated cells. n = 3 independent experiments. B Spearman’s rank correlation analyses showed the correlations between KIAA1429 and ENO1. n = 20 independent experiments. C Correlation between IHC staining intensity for KIAA1429 and ENO1 in tumour sections. n = 20 independent experiments. D LC/MS results showed the m6A methylation level. n = 3 independent experiments. E Dot blot showed changes in m6A methylation. Methylene blue staining was used as a control. n = 3 independent experiments. F The SRAMP database predicted the distribution of m6A sites in ENO1 mRNA. n = 3 independent experiments. G Luciferase activity was measured and normalized to Renilla luciferase activity. n = 3 independent experiments. H MeRip-qPCR analysis of m6A levels in ENO1 mRNA. n = 3 independent experiments. I Changes in ENO1 mRNA stability after ACT-d treatment. n = 3 independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001

Back to article page

Biology Direct

ISSN: 1745-6150

Contact us