Fig. 4

KIAA1429 enhanced aerobic glycolysis on OC cells. A Volcano plot showed the differential genes for RNA-seq. n = 3 independent experiments. B Heatmap showed the 20 most significantly different genes in RNA-seq. n = 3 independent experiments. C KEGG pathway analysis of RNA-seq data. D PCA analysis showed differences between groups. n = 3 independent experiments. E Nontargeted metabolomics GC–MS analysis of glycolysis and TCA cycle metabolites in OC cells. Heatmap showed changes in metabolites of glycolysis or OXPHOS. n = 3 independent experiments. F–I Glucose uptake (F), lactate production (G), pH of the culture medium (H) and OCR (I) were tested in three different cell lines. J The ECAR was measured in three different cell lines using an XF Extracellular Flux Analyzer. n = 3 independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001, and ns indicates no significant difference