Fig. 2

Distribution of Nestin, Dermo1, LeptinR or Gli1-expressing cells in the thymus. (A) Generation of Nestin, Dermo1, LeptinR or Gli1 fluorescent reporter mice. Nestin-Cre, Dermo1-Cre, LeptinR-Cre and Gli-CreERT2 mice were crossed to tdTomato-flox. (B) Nestin, Dermo1, LeptinR or Gli1 (Red) expression respectively in the thymus of Nestin-tdTomato, Dermo1-tdTomato, LeptinR-tdTomato or Gli1-tdTomato transgenic mice (n = 3). Representative immunofluorescence of thymus sections stained for nuclei (Hoechst, blue). (C, D) Nestin (Red) expression in the thymus of Nestin-tdTomato transgenic mice (C, D, n = 3). Representative immunofluorescence of thymus sections stained for Cytokeratin (green) and nuclei (Hoechst, blue). Scale bars, 20 μm, 50 μm and 1 mm. Data are represented as mean ± SEM. ****p < 0.0001