Biological roles of RNA m7G modification and its implications in cancer

Biology Direct

Table 1 Commonly used assays for m7G modification

Technique	Quantitative	Sensitivity	RNA type	Resolution	Main principle	References
Northern blot	yes	low	tRNA	bulk-level RNA	total RNA isolation and RNA blot hybridization	[14]
Primer extension	no	low	rRNA	fragmented RNA	reverse transcription	[15]
LC-MS/MS	yes	low	mRNA, tRNA	bulk-level RNA	liquid chromatography combined with mass spectrometry	[6]
m7G-MERIP-seq	yes	low	mRNA	fragmented RNA	RNA immunoprecipitation	[11]
m7G-miCLIP-seq	yes	high	mRNA	single-base resolution	immunoprecipitation and individual-nucleotide-resolution cross-linking	[4]
m7G-MAP-seq	yes	high	tRNA, rRNA	single-base resolution	chemical reduction and reverse transcription	[18]
m7G-seq	yes	high	mRNA, tRNA	single-base resolution	chemical reduction and reverse transcription	[11]
AlkAniline-seq	yes	high	tRNA, rRNA	single-base resolution	chemical cleavage	[19]
BoRed-seq	yes	high	miRNA	single-base resolution	chemical reduction and reverse transcription	[6]
TRAC-seq	yes	high	tRNA	single-base resolution	chemical cleavage	[17]
m7G-quant-seq	yes	high	tRNA	single-base resolution	chemical reduction and reverse transcription	[20]

ISSN: 1745-6150