Fig. 5

GBAP1 promotes HCC cells metastasis and cell growth in vivo. A and B Hep3B subclones stably overexpressing GBAP1 and or MHCC97H subclones stably expressing shRNA targeting GBAP1 or the control subclones were subcutaneously injected into the flanks of mice. Tumor size was measured every 3 days. Tumor nodules were removed from the flans after 3 weeks and growth curves were constructed. **P < 0.01 (two-way ANOVA with Sidak’s t test). C and D The weight of tumor nodules in different groups was measured. ***P < 0.001 (Student’s t test). E and F RT-qPCR analysis was applied to detect the expression of GBAP1 in mice tumor. ***P < 0.01 (Student’s t test). G and H Immunohistochemical staining was conducted with antibody against Ki-67 in mice tumor slides. ImageJ software was used to value the staining result. Magnification: × 400. **P < 0.01 (Student’s t test). I and J Hep3B subclones stably overexpressing GBAP1 and or MHCC97H subclones stably expressing shRNA targeting GBAP1 or the control subclones were used to constructed lung metastasis model via tail intravenous injection. After 5 weeks, hematoxylin–eosin staining was conducted in the lung tissue slides. ***P < 0.01 (Student’s t test)