Fig. 4

GBAP1 promotes HCC cells migration, invasion and growth in vitro. A pcDNA/GBAP1 was applied to overexpress GBAP1 expression of Hep3B cells. ***P < 0.001 (Student’s t test). B Lentiviral vectors encoding two different shRNAs (shGBAP1#1, shGBAP1#2) that targeted GBAP1 were stably transfected MHCC97H cells. RT-qPCR was used to test the knockdown efficiency. ***P < 0.001 versus shNTC (two-way ANOVA). C and D Transwell assays were used to measure the effects of GBAP1 overexpressing or knockdown on migration and invasion of Hep3B or MHCC97H cells. ***P < 0.001 (Student’s t test). ***P < 0.001 versus shNTC (two-way ANOVA). E and F Wound healing assay was applied to test the effect of GBAP1 overexpressing or knockdown on mobility of Hep3B or MHCC97H cells. ImageJ software was used to value the wound closure. ***P < 0.001 (Student’s t test). ***P < 0.001 versus shNTC (two-way ANOVA). G and H MTT assay was used to detect GBAP1 overexpressing or knockdown on viability of Hep3B or MHCC97H cells. **P < 0.01, ***P < 0.001 (two-way ANOVA with Sidak’s t test). I and J EdU assay was used to test the effects of GBAP1 overexpressing or knockdown on growth of Hep3B or MHCC97H cells. **P < 0.01 (Student’s t test). **P < 0.01, ***P < 0.001 versus shNTC (two-way ANOVA)