Fig. 6
From: Discovery biomarker to optimize obeticholic acid treatment for non-alcoholic fatty liver disease
Effect of OCA on the LX-2 cells transfected with short-interfering RNA (siRNA) againstCYP7B1(si-CYP7B1) and glucose on the expression levels of cytochrome P450 family genes related to bile acid synthesis. (A) Wound healing assay using TGFβ1 and OCA in scramble and si-CYP7B1-transfected LX-2 cells. (B) A wound was introduced in the monolayer of scramble and si-CYP7B1-transfected cells. Wound healing was measured after 24 h. (C) Evaluation of fibrosis-related marker proteins. Comparative analysis of CYP7A1, CYP8B1, and CYP7B1 mRNA levels according to (D) glucose concentrations in HepG2 cells. (E) RORA and NR1H3 mRNA levels according to glucose concentration. Comparative analysis of CYP7A1, CYP8B1, and CYP7B1 mRNA levels according to (F) insulin concentrations in HepG2 cells. Data are shown in bar diagrams as mean ± standard error of mean from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way analysis of variance). Scale bars, 200 μm