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Fig. 3 | Biology Direct

Fig. 3

From: Analysis of state 1—state 2 transitions by genome editing and complementation reveals a quenching component independent from the formation of PSI-LHCI-LHCII supercomplex in Arabidopsis thaliana

Fig. 3

Identification of the PSI-LHCI-LHCII supercomplex induced by PSII-favouring light. The contribution of Lhcb1 and Lhcb2 phosphorylation to the establishment of the PSI-LHCI-LHCII supercomplex was investigated following treatment with PSII-favoring light for 2 h. Isolated thylakoids (35 μg of Chl) were solubilized with 1% (w/v) digitonin and intact protein complexes separated using a non-denaturing a Large Pore Blue Native (lpBN) gel. A dark-adapted (equivalent to state 1) and the kostn7 mutant were employed as negative controls. The PSI-LHCI-LHCII supercomplex was observed in all complemented lines, except for the cB2.1T40V genotype lacking Thr-40 residues, revealing the crucial role of its STN7-dependent phosphorylation in the formation of this complex

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Biology Direct

ISSN: 1745-6150

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