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Fig. 2 | Biology Direct

Fig. 2

From: Analysis of state 1—state 2 transitions by genome editing and complementation reveals a quenching component independent from the formation of PSI-LHCI-LHCII supercomplex in Arabidopsis thaliana

Fig. 2

Immunodecoration of phosphorylated Lhcb1 and Lhcb2 epitopes. The wild type positive control (adapted to state 2, PSII-favoring light) and two negative controls (dark-adapted wild type, equivalent to state 1, and kostn7 mutant) were immunologically probed with α-P-Lhcb1 α-P-Lhcb2 antisera. Panel A: in vivo phosphorylation status of Lhcb1 Thr-38 in the koLhcb1 and Lhcb2 background genotypes and two independent complemented lines carrying the wild type Lhcb1.3 gene copy or the phosphomutant version lacking the phosphorylatable residue (cB1.3 and cB1.3T38V) Panel B: the in vivo phosphorylation status of Lhcb2 Thr-40 in the koLhcb1 and Lhcb2 background genotypes and in two independent complemented lines (cB2.1 and cB2.1T40V). Approximately 0.5 μg of Chl were loaded for each sample

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