Fig. 1

Phenotypes of knockout and complemented genotypes created in this work. A: Genotypes used in this study at six weeks after sowing following cultivation under controlled conditions with a 16/8 h night/day regime under 100 μmol photons m⁻² s⁻¹ light intensity (spectrum given in Additional file 1: Fig. S1). The koLhcb1 mutant exhibited a pale green phenotype while the koLhcb2 genotype was indistinguishable from the wild type plant (upper row). Neither genotype was affected in their growth. The cB1.3 and cB1.3_T38V lines resulting from the complementation of the koLhcb1 genotype recovered the fully green phenotype. B: Immunodecoration with α-Lhcb1 α-Lhcb2 antibodies showing the complete absence of Lhcb1 and Lhcb2 proteins in the genome edited koLhcb1 and koLhcb2 background genotypes