Fig. 7

Down-regulation of circRNA_15430 reduced HP infection-induced in MGC-803 cells. A The expression of LC3 in GES-1 cells and GC cells were detected by Western Blotting B The expression of circRNA_15430 was detected by qRT-PCR when MGC-803 cells were treated with different concentrations of Rapamycin. C The number of autophagosomes was detected by confocal laser microscopy when circRNA_15430 was knocked down in MGC-803 cells (scale bars = 25 μm). D After HP26695 infected MGC-803 cells at a MOI = 50:1 for different times (0, 4, 8, and 12 h), the relative expression of circRNA_15430 was assessed by qRT-PCR. E After HP26695 infected MGC-803 cells with different MOIs (0, 50 and 100) for 4 and 8 h, the expression of circRNA_15430 was detected by qRT-PCR. F qRT-PCR monitored the expression of circRNA_15430 in HP + gastritis tissues (n = 12) and HP- gastritis tissues (n = 19). G confocal laser microscopy analyzed the number of autophagosomes when HP26695 was infected with the circRNA_15430 knockdown MGC-803 cells (scale bars = 25 μm). H The expression level of miR-382-5p in MGC-803 cells infected with HP for 12 h with different MOI values (MOI = 25, 50 and 100) by qRT-PCR. I The expression level of miR-382-5p in HP + gastritis tissues (n = 12) and HP- gastritis tissues (n = 19) were detected by qRT-PCR. J The expression level of ZCCHC14 in MGC-803 cells infected with HP for 12 h with different MOI values (MOI = 25, 50 and 100) by qRT-PCR. K The number of autophagosomes was detected by confocal laser microscopy when the expression of ZCCHC14 and miR-382-5p was decreased and LHP26695 infected MGC-803 cells for 12 h with MOI = 50 (scale bars = 50 μm). *P < 0.05, **P < 0.01, ***P < 0.001