Fig. 5

MiR-382-5p targets ZCCHC14 and regulates the progression of gastric cancer. A Three public database predicted the genes targeted by miR-382-5p. B qRT-PCR detected the effect of abnormal miR-382-5p expression on the expression of ZCCHC14 in GC cells. C The expression levels of ZCCHC14 in GES-1 cells and four GC cells were detected by qRT-PCR. D Kaplan-Meier Plotter database was used to analyze the relationship between ZCCHC14 expression and OS, DFS, and PFS in GC patients. E qRT-PCR was used to detect the effect of si-ZCCHC14 on the expression of ZCCHC14 in HGC-27 cells. F Transwell migration assay, G CCK-8 assay, H Flow cytometry assay detected the effect of ZCCHC14 knockdown on migration, growth and cell apoptosis in HGC-27 cells. I CCK-8 assay, J Transwell migration assay, and K colony formation assay detected the effect of si-ZCCHC14 and miR-382-5p inhibitor co-transfected into HGC-27 cells on cell migration and proliferation. L qRT-PCR detected the expression of ZCCHC14 when knockdown and overexpress circRNA_15430 in GC cells. M qRT-PCR detected the expression of circRNA_15430 when knockdown ZCCHC14 in HGC-27 cells. N CCK-8 assay, O flow cytometry assay, and P Transwell migration assay examined the function of co-transfection of circRNA_15430 plasmids and ZCCHC14 siRNA in HGC-27 cells on cell growth, apoptosis and metastasis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001