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Fig. 1 | Biology Direct

Fig. 1

From: MAP7D3, a novel prognostic marker for triple-negative breast cancer, drives cell invasiveness and cancer-initiating cell properties to promote metastatic progression

Fig. 1

Identification of isogenic pair of TNBC cell lines with low and high motility. (A) Cell morphology of MDA-MB-231-P and IV2 cells under light microscopy (100X). The lower panel is the enlargement of single cell image from the upper panel image. (B) Cell morphology of MDA-MB-468-P and 468-LN cells under light microscopy (100X). The lower panel is the enlargement of single cell image from the upper panel image. (C) Time lapse of wound healing assay. Scale bar, 500 μm. (D) Quantitative results of migrating area between 231-P and IV2. (E) Time lapse of wound healing assay. Scale bar, 500 μm. (F) Quantitative results of migrating area between 468-P and 468-LN. *** P < 0.001. (G) qRT-PCR analysis of MAP7, MAP7D1, MAP7D2, MAP7D3 in 231-P and IV2. (H) Western blotting analysis of MAP7D1, MAP7D2 and MAP7D3 in 231-P and IV2. (I) qRT-PCR analysis of MAP7, MAP7D1, MAP7D2, MAP7D3 in 468-P and 468-LN. (J) Western blotting analysis of MAP7D1, MAP7D2 and MAP7D3 in 468-P and 468-LN. *** P < 0.001. *P < 0.05. Each experiment was performed in triplicate and repeated three times

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