Fig. 2

Ultrasound examination after Peroneus tertius muscle surgical removal (3 × 2 × 1 cm) (n = 5). (A). Representative eco-graphic images of ablated (ABL) and healthy (CTRL) muscles analyzed at 30, 60, 90, 150 and 180 days after ablation. The removed zone appears as a black area delimited in the pictures by white crosses, while the orderly muscle fibers populate the white area. (B). Violin plot showing the Mean Grey Value calculated from ultrasound images for each time points to evaluate lesion filling. The violin plot quantitated the sum of the gray values of all the pixels in the selection divided by the number of pixels: reported in calibrated units (e.g., optical density). For each time point n = 10 ultrasound images were analyzed (n = 2 for each animal) and the average value of the MGV was graphed. One way ANOVA Tukey’s Multiple Comparison test was performed. Scale bars: CTRL = 2 cm; ABL = 1 cm.