Fig. 6

Hsa_circ_0067842 inhibits the degradation of PD-L1 via HuR/CMTM6 axis. A Western blot assays of CMTM6 expression in si-NC- or si-CMTM6-expressing MCF-7 or BT-549 cells. B Western blot assays of CMTM6 expression in si-NC- or si-HuR-expressing MCF-7 or BT-549 cells. C Western blot assays of CMTM6 expression in hsa_circ_0067842- or si-hsa_circ_0067842-expressing BC cells. D Ubiquitination level of PD-L1 in MCF-7 cell was detected via immunoprecipitation and western blot after knocking down CMTM6. E Ubiquitination level of PD-L1 in MCF-7 cell was detected via immunoprecipitation and western blot after knocking down HuR. F Ubiquitination level of PD-L1 in MCF-7 cell was detected via immunoprecipitation and western blot after overexpressing hsa_circ_0067842. G Protein expression of PD-L1 and CMTM6 was detected via western blot. H Ubiquitination level of PD-L1 in MCF-7 cell was detected via rescue experiments