Fig. 4

PHF5A promotes the proliferation of HNSCC

(A) CCK-8 assays showed the proliferation of cancer cells after knockdown of PHF5A expression by two siRNAs in JHU011 and Tu686 cells. (B) Colony formation assays showed that colony formation was largely inhibited after knockdown of PHF5A expression in JHU011 and Tu686 cells. (C, D) CCK-8 (C) and colony formation assays (D) demonstrated that proliferation and colony formation were enhanced following overexpression of PHF5A in FaDu cells. (E, F) After overexpression of PHF5A in mouse MEER cells, CCK-8 (E) and colony formation assays (F) demonstrated that cell proliferation and colony formation were enhanced. (G) The final gross tumours, tumour volume and tumour weight are shown in the subcutaneous xenograft mouse model. (H) Representative immunohistochemical staining for the Ki-67 protein in the tumours of the subcutaneous xenograft mouse model. ***: P < 0.001, **: P < 0.01, *: P < 0.05. NC, negative control; EV, empty vector group; OE, PHF5A overexpression group