Fig. 4
From: Pro-tumorigenic role of lnc-ZNF30-3 as a sponge counteracting miR-145-5p in prostate cancer
MiR-145-5p specifically binds to lnc-ZNF30-3 in PC3a cells. A. Sequences of miRNA mimics (5’ -3’): wt – wild-type consensus of miR-145-5p, mut – mutated sequence containing two nucleotide substitutions (underlined), pas – passenger miR-145-3p strand. B. Dot blot analysis of biotinylated (+) and non-biotinylated (-) miR-145-5p detected in PC3a lysates 24 h post-transfection in pellet (cell debris), input (total cell lysate), FT (flow through) and pull-down (streptavidin beads). C. Western blot analysis of AGO2 precipitated by biotinylated (+) or non-biotinylated (-) miR-145-5p primed streptavidin beads; ACT1 was used as a negative control. D. RT-qPCR quantification of TWIST1 and ZEB2 mRNAs, and lnc-ZNF30-3 co-precipitated by streptavidin beads, primed with biotinylated miR-145-5p wt, mut, and scr sequences.