Fig. 2

Identification of PCa-associated lncRNAs containing REs and interacting with miR-145 in PCa cells. A. Summary table representing different types of PCa-associated lncRNAs and a number of RE for the canonical miR-145-5p seed motif (UCCAGU) identified by bioinformatics analysis of seed match. As-lncRNA – antisense lncRNA, so-lncRNA – sense overlapping lncRNA. B. 2D plot of miR-145-5p conventional RE sequence occurrence and the respective fold- change of lncRNA expression in prostate cancer (n = 16) compared with normal tissues (n = 8). LncRNA annotation includes long intergenic noncoding (linc)RNAs and antisense lncRNAs (GENCODE v26). LncRNAs associated with PCa in previous studies are labeled in red; lnc-ZNF30-3 is labeled in black. C-D. RT-qPCR quantification of lncRNAs co-precipitated with biotin-labeled miR-145 mimics on streptavidin beads from PC3a (C) and 22Rv1 (D) cells. E. Enrichment of lnc-ZNF30-3 in PCa cell lines LNCaP, 22Rv1, DU145, PC3a, and immortalized normal prostate cell line PNT1A normalized by PNT1A presented in log2 scale.