Fig. 9
PSMA3-AS1 knockdown inhibits tumor growth in nude mice. (A) The nude mice were subcutaneously injected with 1 × 106 stable transfectants. On day 32, the mice were euthanatized. The tumors were excised and photographed. (B) Tumor volume was monitored every 4 days (n = 6). (C) The tumors were weighed (n = 6). (D) Total RNAs were isolated from tumor tissues. PSMA3-AS1, miR-329-3p, and ALDOA levels in tumor tissues were determined by real-time PCR (n = 3). GAPDH was used as an internal control for lncRNA and mRNA, and U6 was for miRNA measurement. (E) Total proteins were extracted from tumor tissues. ALDOA, MMP-2, and MMP-9 in tumor tissues were measured by western blotting (n = 3). GAPDH served as an internal control. (F) MDA content, SOD activity, and GSH-Px activity in tumor tissues were measured (n = 3). MDA content is expressed as nmol/mg prot. SOD activity is expressed as U/mg prot. GSH-Px activity is expressed as U/mg prot. (G) The nude mice were subcutaneously injected with 1 × 106 stable transfectants. On day 32, the mice were euthanatized. The tumors were excised and photographed. Tumor volume was monitored every 4 days (n = 6). (H) The tumors were weighed (n = 6). *P < 0.05 and **P < 0.01 compared with sh-NC or vector. Student′s t-test was used to compare two groups, and one-way ANOVA with Tukey′s post-hoc test was used to compare multiple groups