Fig. 8

PSMA3-AS1 knockdown exerts its anti-tumor function in GC cells by targeting ALDOA. (A) AGS cells stably knocked down PSMA3-AS1 were transfected with ALDOA overexpression plasmid or vector. Real-time PCR was performed to determine ALDOA expression in AGS cells (n = 3). GAPDH served as an internal control. (B) After transfection, total proteins were extracted, and western blotting was conducted to determine ALDOA expression in AGS cells (n = 3). GAPDH served as an internal control. (C) After transfection, cell proliferation was evaluated by CCK-8 assay (n = 3). (D) After transfection, cell migration was assessed by wound healing assay (n = 5). (E) After transfection, cell invasion was assessed by transwell assay (n = 5). (F) After transfection, cell apoptosis was evaluated by Annexin V-FITC/PI assay (n = 3). (G) After transfection, intracellular ROS level was analyzed. (H) After transfection, MDA content, SOD activity, and GSH-Px activity were determined (n = 3). ^**P < 0.01 compared with sh-NC + vector. ^#P < 0.05 and ^##P < 0.01 compared with sh-PSMA3-AS1 + vector. One-way ANOVA with Tukey′s post-hoc test was used to compare multiple groups