Fig. 6

PSMA3-AS1 knockdown exerts its anti-tumor function in GC cells by regulating miR-329-3p. (A) AGS cells stably knocked down PSMA3-AS1 were transfected with miR-329-3p inhibitor or NC inhibitor. Cell proliferation capacity was measured by CCK-8 assay (n = 3). (B) After transfection, cell migration ability was evaluated by wound healing assay (n = 5). (C) After transfection, cell invasion ability was assessed by transwell invasion assay (n = 5). (D) After transfection, cell apoptosis was evaluated (n = 3). (E) After transfection, intracellular ROS level was examined using DCFH-DA fluorescent probes (n = 3). (F) After transfection, MDA content, SOD activity, and GSH-Px activity were measured (n = 3). MDA content is expressed as nmol/mg prot. SOD activity is expressed as U/mg prot. GSH-Px activity is expressed as U/mg prot. ^**P < 0.01 compared with sh-NC + NC inhibitor. ^#P < 0.05 and ^##P < 0.01 compared with sh-PSMA3-AS1 + NC inhibitor. One-way ANOVA with Tukey′s post-hoc test was used to compare multiple groups