Fig. 4

PSMA3-AS1 knockdown activates oxidative stress in GC cells. (A) Intracellular ROS level was determined using DCFH-DA fluorescent probes, and the results were read on a fluorescence plate reader (n = 3). (B) MDA content, SOD activity, and GSH-Px activity were examined (n = 3). MDA content is expressed as nmol/mg prot. SOD activity is expressed as U/mg prot. GSH-Px activity is expressed as U/mg prot. ^*P < 0.05 and ^**P < 0.01 compared with sh-NC or vector. Student′s t-test was used to compare two groups