Fig. 3

PSMA3-AS1 knockdown promotes GC cell apoptosis. (A) Cell apoptosis was evaluated by flow cytometry (n = 3). The apoptotic rate was determined by counting early (Annexin V⁺/PI^–) and late apoptotic rates (Annexin V⁺/PI⁺). (B) Total proteins were extracted from stable transfectants. The levels of Bax, Bcl-2, cleaved caspase-3, and cleaved PARP in AGS and HGC-27 cells were determined by western blotting (n = 3). GAPDH served as an internal control. ^**P < 0.01 compared with sh-NC or vector. Student′s t-test was used to compare two groups