Fig. 1

PSMA3-AS1 is upregulated in GC tissues and cell lines. (A) Human fresh tumor tissues and adjacent nontumorous tissues were obtained from patients with GC. PSMA3-AS1 levels in 20 paired clinical specimens were examined by real-time PCR (n = 20). GAPDH served as an internal control. (B) PSMA3-AS1 levels in four GC cell lines and GES-1 cells were determined by real-time PCR (n = 3). GAPDH served as an internal control. ^**P < 0.01 compared with Normal or GES-1 cells. Student′s t-test was used to compare two groups, and one-way ANOVA with Tukey′s post-hoc test was used to compare multiple groups