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Fig. 3 | Biology Direct

Fig. 3

From: Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathway

Fig. 3

Effects of over-expressed IGFBP3 on odontogenic and osteogenic differentiation of human dental papilla stem cells and dental mesenchyme. A IGFBP3 expression in lv5 hDPSCs versus IGFBP3-over hDPSCs after lentivirus transfection (a); Relative expression of DSPP, DMP1, OSX, OPN, OCN and ALP in lv5 hDPSCs versus IGFBP3-over hDPSCs after 0 days (b), 4 days (c) and 7 days (d) osteogenic induction. B The IGFBP3 expression response to osteogenic induction was investigated by RT-qPCR using hDPSCs. C The protein levels of IGFBP3, odontogenic and osteogenic markers in lv5 hDPSCs and IGFBP3-over hDPSCs were detected by Western blot analysis (a). Quantitative analysis of the relative protein expression in IGFBP3 (b1), DSPP (b2), OSX (b3), OPN (b4), OCN (b5) and ALP (b6). D Alizarin red staining of lv5 hDPSCs and IGFBP3-over hDPSCs with 14 days osteogenic induction; Alkaline phosphatase staining of lv5 hDPSCs and IGFBP3-over hDPSCs treated with osteogenic induction medium for 7 days. Microscopic views: a1–d1, scale bar 200 μm; gross views: a2–d2. E Microscopic views of dental mesenchymal tissue from the P1 tooth germ with lentivirus transfection (a). Relative expression of Igfbp3, Dspp, Ocn in lv5 dental mesenchyme and IGFBP3-over dental mesenchyme were detected by RT-qPCR (b). lv5, hDPSCs or dental mesenchyme with empty pGLV5 vector control; over, hDPSCs or dental mesenchyme with IGFBP3 overexpressing vector. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001

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Biology Direct

ISSN: 1745-6150

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